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KMID : 0355420020260040511
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Journal of Korean Academy of Oral Health
2002 Volume.26 No. 4 p.511 ~ p.521
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Cloning of DNA probes for Campylobacter rectos ATCC 33238
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Abstract
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The purpose of this study is to develop strain-specific DNA probes and polymerase chain reaction (PCR) primers for detection and identification of Camphyrobactor rectus (C, rectus) ATCC 33238.
This study procedure included (1) whole-genomic DNA extraction of C. rectus ATCC 33238, (2)
:.construction of the genomic DNA library, (3) screening of restriction, fragment of genomic DNA by reverse dot hybricii7ation, (4) isolation of strain-specific DNA probes by Southern blot hybridization. Thirty-five restriction fragments of C. rectus ATCC 33238 genomic DNA digested with the Hind III were obtained.
Southern blot analysis data showed that two of them, Cr/l4 3 (832 bp) and Cr44-4 (669 bp) were C. rectos ATCC 33238 specific DNA probes. Our data indicate that these DNA probes may be useful in detection and identification of the C. rectus ATCC 33238.
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